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Asociacion de variante RS2910164 en el gen miR-146A con “Obeso metabolicamente saludable”.
Penas Steinhardt, Alberto

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Cambios en la Expresion interleuqina 1BETA en pacientes con DBT tipo 2 descompensada metabolicamente y luego del tratamiento y su asociacion con SNPS y cambios epigeneticos
Penas Steinhardt, Alberto

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Getting to Know the Gut Microbial Diversity of Metropolitan Buenos Aires Inhabitants
Penas Steinhardt, Alberto

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Impact of the use of biologics and their discontinuation on the gut microbiota of psoriasis patients
Penas Steinhardt, Alberto

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Metabolically healthy obese individuals present similar chronic inflammation level but less insulin-resistance than obese individuals with metabolic syndrome
Penas Steinhardt, Alberto

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Título:	Metabolically healthy obese individuals present similar chronic inflammation level but less insulin-resistance than obese individuals with metabolic syndrome
Autor(es):	Penas Steinhardt, Alberto
Descripción:	Fil: Penas Steinhardt, Alberto. Instituto Universitario de Ciencias de la Salud – Fundación Barceló. Secretaria de Ciencia y Tecnologia; Argentina.
Resumen:	The Metabolic Syndrome (MetS) is a cluster of cardiometabolic risk factors, usually accompanied by the presence of insulin resistance (IR) and a systemic subclinical inflammation state. Metabolically healthy obese (MHO) individuals seem to be protected against cardiometabolic complications. The aim of this work was to characterize phenotypically the lowgrade inflammation and the IR in MHO individuals in comparison to obese individuals with MetS and control non obese. We studied two different populations: 940 individuals from the general population of Buenos Aires and 518 individuals from the general population of Venado Tuerto; grouped in three groups: metabolically healthy non-obese individuals (MHNO), MHO and obese individuals with MetS (MSO). Inflammation was measured by the levels of hs-CRP (high-sensitivity C reactive protein), and we found that MHO presented an increase in inflammation when compared with MHNO (Buenos Aires: p<0.001; Venado Tuerto: p<0.001), but they did not differ from MSO. To evaluate IR we analyzed the HOMA (Homoeostatic Model Assessment) values, and we found differences between MHO and MSO (Buenos Aires: p<0.001; Venado Tuerto: p<0.001), but not between MHNO and MHO. In conclusion, MHO group would be defined as a subgroup of obese individuals with an intermediate phenotype between MHNO and MSO individuals considering HOMA, hs-CRP and central obesity.
Descriptores:
Fecha:	2020-02-27
Formato:	application/pdf
Extensión:	11 paginas
Idioma:	eng
Lugar:	7006287, Buenos Aires (inhabited place)
Sede:	Buenos Aires
Carrera:	MEDICINA
Notas:	Trabajo de investigación publicado

Metagenomic analysis of gut microbiota in non‑treated plaque psoriasis patients stratified by disease severity: development of a new Psoriasis‑Microbiome Index
Penas Steinhardt, Alberto

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Título:	Metagenomic analysis of gut microbiota in non‑treated plaque psoriasis patients stratified by disease severity: development of a new Psoriasis‑Microbiome Index
Autor(es):	Penas Steinhardt, Alberto
Descripción:	Fil: Penas Steinhardt, Alberto. Instituto Universitario de Ciencias de la Salud – Fundación Barceló. Secretaria de Ciencia y Tecnologia; Argentina.
Resumen:	Psoriasis is an immune-mediated skin disorder. Imbalance of gut microbial populations has been implicated in many diseases. We aimed to investigate whether there were differences in gut microbiota in psoriasis patients vs non-psoriasis controls and between psoriasis severity groups. 55 psoriasis patients and 27 controls were included. V3–V4 regions of the 16S rRNA gene of fecal samples were analyzed using Illumina MiSeq. Bioinformatic analysis was performed. We found changes in gut microbiome composition depending on their psoriasis status as determined by weighted unifrac (p < 0.05), in particular an increase in Firmicutes and depletion of Bacteroidetes in psoriasis patients. Additionally, the Faecalibacterium and Blautia genus were higher in psoriasis patients while Bacteroides and Paraprevotella in non-psoriasis controls (p < 0.05, LDA score > 2). Moderate-to-severe psoriasis patients had lower biodiversity than mild psoriatic patients (p = 0.049). No differences for beta-diversity were found. We developed a Psoriasis-Microbiota Index (PMI), which discriminated among psoriasis patients and controls with sensitivity: 0.78 and specificity: 0.79. Furthermore, we performed a meta-analysis with published data to validate this index. We demonstrated gut dysbiosis in psoriasis patients, suggesting a role in psoriasis pathophysiology. Furthermore, we developed a PMI with the potential to discriminate between psoriasis patients and controls across different populations, which could be used as a biomarker in the clinical practice.
Descriptores:
Fecha:	2020-09-10
Formato:	application/pdf
Extensión:	11 paginas
Idioma:	eng
Lugar:	7006287, Buenos Aires (inhabited place)
Sede:	Buenos Aires
Carrera:	MEDICINA
Notas:	Proyecto de investigación

Metagenomic analysis of the human gut microbiome in Inflammatory Bowel Disease
Penas Steinhardt, Alberto

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Título:	Metagenomic analysis of the human gut microbiome in Inflammatory Bowel Disease
Autor(es):	Penas Steinhardt, Alberto
Descripción:	Fil: Penas Steinhardt, Alberto. Instituto Universitario de Ciencias de la Salud – Fundación Barceló. Secretaria de Ciencia y Tecnologia; Argentina.
Resumen:	Gut microbiota is implicated in many human disorders. Ulcerative colitis (UC) is a chronic inflammatory bowel disease. Although the specific cause is unknown, some genetic and environmental factors have been defined. The purpose of this study was to investigate whether there were differences in gut microbiota in Argentine UC patients vs non-UC controls. In this sense, 23 UC patients and 27 non-UC healthy controls matched by sex, age and BMI were included. DNA extraction was performed from 200mg of feces using Power Fecal DNA Isolation Kit (Qiagen).The hypervariable regions V3-V4 of the bacterial 16S gene was sequenced using MiSeq-Illumina system. Sequences generated were analyzed using quantitative insights into microbial ecology (QIIME) version 1.9.1 software package. To compare taxa relative abundance between groups, we performed linear discriminant analysis (LDA) effect implemented in LEfSe. Alfa diversity did not differ between CU patients and non-CU controls. However, we found that CU patients differ from non-CU controls in the observed community structure. In CU patients, the dominant phyla were Bacteroidetes (43.49±20.18%), Firmicutes (48.94±18.61%), Proteobacteria (4.13±7.12%), Actinobacteria (2.12±1.97%) and Verrucomicrobia (0.38±1.01%) while the principal phyla found in Controls were Bacteroidetes (60.06±13.540%), Firmicutes (32.82±13.51%), Proteobacteria (4.27±3.32%), Verrucomicrobia (1.45±3.18%) and Actinobacteria (0.81±1.46%).The linear discriminant analysis (LDA) effect size (LEfSe) method revealed that the genus Bacteroides and Akkermansia were higher in non-CU control and Bifidobacterium, Eubacterium, Lactobacillus, Collinsella, Peptostreptococcus, Actinomyces, Streptococcus, Slackia and Dialister in CU patients (p < 0.05, LDA score > 2).
Descriptores:
Fecha:	2020-09-10
Formato:	application/pdf
Extensión:	1 pagina
Idioma:	eng
Lugar:	7006287, Buenos Aires (inhabited place)
Sede:	Buenos Aires
Carrera:	MEDICINA
Notas:	Proyecto de investigación

Metagenómica en enfermedades inflamatorias intestinales crónicas.
Penas Steinhardt, Alberto

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Registro del documento
Título:	Metagenómica en enfermedades inflamatorias intestinales crónicas.
Autor(es):	Penas Steinhardt, Alberto
Descripción:	Fil: Penas Steinhardt, Alberto. Instituto Universitario de Ciencias de la Salud – Fundación Barceló. Secretaria de Ciencia y Tecnologia; Argentina.
Resumen:	La microbiota intestinal está implicada en diversas patologias humanas. La colitis ulcerosa (CU) es una enfermedad inflamatoria intestinal crónica. Aunque se desconoce la causa específica, se han definido algunos factores genéticos y ambientales. El propósito de este estudio fue investigar si había diferencias en la microbiota intestinal en pacientes argentinos con CU versus controles sin UC. En este sentido, se incluyeron 23 pacientes con CU y 27 controles sanos sin CU, emparejados por sexo, edad e IMC. La extracción de ADN se realizó a partir de 200 mg de heces usando el kit PowerFecalDNAIsolation (Qiagen). Las regiones hipervariables V3-V4 del gen bacteriano 16S se secuenciaron usando el sistema MiSeq-Illumina. Las secuencias generadas se analizaron utilizando el conocimiento cuantitativo de la ecología microbiana (QIIME) versión 1.9.1 del paquete de software. Para comparar la abundancia relativa de taxones entre grupos, realizamos un efecto de análisis discriminante lineal (LDA) implementado en LEfSe. La diversidad alfa no difirió entre los pacientes con CU y los controles sin CU. Sin embargo, encontramos que los pacientes con CU difieren de los controles sin CU en la estructura comunitaria observada. En pacientes con CU, los filamentos dominantes fueron Bacteroidetes (43.49 ± 20.18%), Firmicutes (48.94 ± 18.61%), Proteobacterias (4.13 ± 7.12%), Actinobacterias (2.12 ± 1.97%) y Verrucomicrobia (0.38 ± 1.01%), mientras que las principales Los phyla encontrados en los controles fueron Bacteroidetes (60.06 ± 13.540%), Firmicutes (32.82 ± 13.51%), Proteobacterias (4.27 ± 3.32%), Verrucomicrobia (1.45 ± 3.18%) y Actinobacterias (0.81 ± 1.46%). El análisis discriminante lineal ( El método de tamaño del efecto LDA (LEfSe) reveló que los géneros Bacteroides y Akkermansia fueron más altos en el control sin CU y Bifidobacterium, Eubacterium, Lactobacillus, Collinsella, Peptostreptococcus, Actinomyces, Streptococcus, Slackia y Dialister en pacientes con CU (p <0.05, puntaje LDA > 2). Nuestros resultados demostraron que había diferencias en la microbiota intestinal en pacientes con CU. Estos hallazgos podrían proporcionar las bases para comprender mejor la relación causa-efecto entre comunidades microbianas y CU en la población argentina.
Descriptores:
Contribuidores:	Frechtel, Gustavo D.
Fecha:	2020-10-14
Formato:	application/pdf
Extensión:	22 paginas
Idioma:	spa
Lugar:	7006287, Buenos Aires (inhabited place)
Sede:	Buenos Aires
Carrera:	MEDICINA
Notas:	Trabajo de investigación publicado

New insights in Ulcerative Colitis Associated Gut Microbiota in South American Population: Akkermansia and Collinsella , two distinctive genera found in Argentine subjects.
Penas Steinhardt, Alberto

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